By Henry F. Epstein and Diane C. Shakes (Eds.)
The 1st of its sort, this laboratory guide emphasizes assorted equipment and applied sciences had to examine C. elegans, either as an built-in organism and as a version procedure for learn inquiries in cell,developmental, and molecular biology, in addition to in genetics and pharmacology. 4 basic sections--Genetic and tradition tools, Neurobiology, mobilephone and Molecular Biology, and Genomics and Informatics--reflect the cross-disciplinary nature of C. elegans study. simply because C. elegans is an easy and malleable organism with a small genome and few telephone varieties, it offers a chic demonstration of capabilities basic to multicellular organisms. The self-discipline has drastically accelerated as researchers proceed to discover this small soil nematode to be the version of selection for learning particular pathways, phases of improvement, and telephone varieties. by means of directing its viewers not only to tried-and-true recipes for learn, but additionally to databases and different leading edge assets of data, this accomplished assortment is meant to steer investigators of C. elegans for years yet to come.
First single-source ebook detailing motives of present and vintage C. elegans methodologies
Diversity and scope of thoughts coated anticipated to be worthy to the broadening neighborhood of C. elegans researchers for years to come
Techniques variety from opposite genetics and mutagenesis, to laser ablation and electrophysiology, to in situ hybridization and DNA sequencing methods
Appendices contain source info vital to the C. elegans group, together with the C. elegans Genetics middle and net assets just like the malicious program group process and ACeDB
Illustrated with greater than a hundred tables and figures
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Extra resources for Cuenorhubditis elegans: Modern Biologcal Analysis of an Organism
Cox, G. , Laufer, J. , and Edgar, R. S. (1980). Genetic and phenotypic characterization of roller mutants of Caenorhabditis elegans. Genetics 95,317-339. Cox, G. , and Edgar, R. S. (1981a). The cuticle of Caenorhabditis elegans. 11. Statespecific changes in ultrastructure and protein composition during postembryonic development. Dev. Biol. 86,456-470. Cox, G. , and Edgar, R. S. (1981b). Temporal regulation of cuticle synthesis during development of Caenorhabditis elegans. Dev. Biol. 84,277-285.
What is the frequency of such events following mutagenesis? The second is mutagen specificity. )? The goals of a mutagenesis protocol are often conflicting. A very potent mutagen is of little value if it does not increase the frequency of the desired type of lesion. For example, ethyl methanesulfonate is an exceedingly potent mutagen, but almost all of its mutational products are G/C + A/T transitions. Ethyl methanesulfonate is worthless if the goal is to isolate transposon insertions. Conversely, a very specific mutagen may not be helpful if it is inefficient.
Proc. Natl. Acad. Sci. A. 76,1333-1337. Epstein, H. , Waterston, R. , and Brenner, S. (1974). A mutant affecting the heavy chain of myosin in Caenorhabditis elegans. J. Mol. Biol. 90,291-300. Garcea, R. , and Epstein, H. F. (1978). Coordinate synthesis of two myosins in wildtype and mutant nematode muscle during larval development. Cell 15,421-428. , Rohner, S. , and Buckland, B. (1994). Large scale cultivation of the free living nematode Caenorhabditis elegans. Biotechnology 12,5144. Golden, J.