The Mammalian Egg Coat: Structure and Function by M. E. Chamberlin, M. J. Ringuette, C. C. Philpott, S. M.

By M. E. Chamberlin, M. J. Ringuette, C. C. Philpott, S. M. Chamow (auth.), Priv.-Doz. Dr. med Johannes Dietl (eds.)

Publication of this monograph recognizes nice strides made in examine at the zona pellucida prior to now decade or so. The physique of labor provided testifies to a resurgence of curiosity within the zona pellucida, caused principally by way of a renewed appreciation of its distinctive homes and its vital features in the course of early mam­ malian improvement. it truly is enjoyable to achieve that the zona pellucida has finally completed a genuinely-earned awareness. while extracellular coats of nonmammalian eggs were studied intensively for a few years, the zona pellucida by means of comparability, has formerly taken a again seat. thankfully, the learn offered right here indications an finish to this situ­ ation. a few of the problems inherent in operating with fairly small quantities of fabric were triumph over, paving the best way for program of a greater diversity of experimental ways to the zona pellucida by means of a bigger neighborhood of investigators. with a bit of luck, this monograph will function a catalyst during this regard. As published in those pages, software of latest experi­ psychological methodologies has had major effect on either natural and utilized learn at the zona pellucida. for instance, modem im­ munological ways were used to evaluate molecular fea­ tures of zona pellucida constitution and serve as, in addition to to evalu­ ate the zona pellucida as a possible contraceptive goal antigen.

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The appearance of the repeat depended upon the way in which filaments were pre- 27 "he Mouse Egg Receptor For Sperm ". _. B . c - . - -- . /""::: .. : . ' ~ - . <~~:~ Fig. 8. Electron micrographs of mouse ZP filaments, sprayed onto mica-coated grids, and platinum/ carbon shadowed. Filaments were prepared by solubilizing isolated ZP with elastase (see (20)). The filament shown in panel B is more than 2 ~m in length. Note the interconnection between filaments in panel C (indicated by white bars) pared for EM.

It is further observed that two different ZP antigens are expressed if cultured on different extracellular matrices. Indirect evidence that granulosa cells produce the ZP matrix also exists. When rabbits are immunized with ZP proteins, the oocytes are destroyed at the stage at which they begin to initiate formation of the zona pellucida matrix (see discussion Ovarian Development 45 Fig. 6 A-D. Immunocytochemical localization in frozen sections of 6-week-old rabbit ovaries of ZP antigens. At this age, most of the follicles are growing, although they will eventually undergo atresia.

The dual role of ZP3, as receptor and structural element, is not unique in the context of extracellular matrix components of somatic cells. Structure and Expression of the Sperm Receptor We found that isolated growing oocytes, denuded of follicle cells and cultured in vitro in the presence of [35 s]methionine or [3H]fucose, synthesized and secreted all three ZP glycoproteins [8,13,21 - 24]. Accordingly, pulse-chase experiments utilizing either enzymes or chemical agents that remove N- and/ or O-linked oligosacchari des from glycoproteins, and experiments carried out in the presence of tunicamycin to prevent N-linked glycosylation of nascent proteins, permitted construction of a biosynthetic pathway for ZP3 (Fig.

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